Alkaline extract from vermicompost reduced the stress promoted by As on maize plants and increase their phytoextraction capacity.

Arsenic (As) represents an environmental risk and phytoremediation has been identified as a good technique to recover contaminated soils. Plants defense mechanisms needed to be enhanced against As stress-promoting action by biostimulants such as humic materials. This work sought to determine the effectiveness of an alkaline vermicompost extract (AEV) and in mitigating stresses promoted by As in maize plants, increasing their potential use for phytoextraction. The AEV were extracted from vermicompost and two preliminary assays in Leonard pots were carried out: the first one to define the best AEV concentration-response dose and the second to point out the toxic As concentration. The second step was to set up a 28-day long experiment with the following four treatments: control, AEV, As, As + AEV. AEV attenuated As-induced stress in maize plants. Maize dry biomass was reduced in the As treatment and rebalanced to values similar to the control in the As + HS treatment while the plants treated only with HS showed the highest biomass among the treatments. The concentrations of P, Fe, Cu, Mn and Ni, and catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD) antioxidant activity increased in the As treatment and decreased in the As + AEV treatment. The rate of photosynthesis decreased, and the internal CO2 concentration increased with stress induced by As, where both effects were attenuated by AEV. Our results show the positive effect of the AEV in alleviating As abiotic stress on maize growth, offering new options of employment of humic substances in phytoremediation process.

Effects of different humic substances concentrations on root anatomy and Cd accumulation in seedlings of Avicennia germinans (black mangrove).

Mangrove areas are among most threatened tropical ecosystems worldwide. Among polluting agents Cadmium is often found in high concentrations in mangrove sediments. Humic substances, complex biomolecules formed in soil and sediments during animal and plant residuals decomposition, have a known biostimulant activity and can be adopted to counteract various plant stresses. This study explores, in controlled conditions, the effect of humic substances on Avicennia germinans seedlings, with or without cadmium contamination. Humic compounds significantly changed plant root architecture, and, when coupled with cadmium, root anatomy and Cortex to Vascular Cylinder diameter ratio. These modifications led to lower Cd uptake by humic substances-treated plants. Humic substances amendment could be effective, depending on their concentrations, on improving plant health in mangrove areas, for forest recuperation and/or dredged sediments phytoremediation purposes.

Alleviation of iron toxicity in Schinus terebinthifolius Raddi (Anacardiaceae) by humic substances.

One of the industrial pillars of Espírito Santo state, South East of Brazil, is iron-mining products processing. This activity brings to a high level of coastal pollution due to deposition of iron particulate on fragile ecosystems as mangroves and restinga. Schinus therebinthifolius (aroeira) is a widespread restinga species. This work tested iron toxicity alleviation by vermicompost humic substances (HS) added to aroeira seedlings in hydroponic conditions. Catalase, peroxidase, and ascorbate peroxidase are antioxidant enzymes that work as reactive oxygen species (ROS) scavengers: they increase their activity as an answer to ROS concentration rise that is the consequence of metal accumulation or humic substance stimulation. S. terebinthifolius seedlings treated with HS and Fe augmented their antioxidant enzyme activities significantly less than seedlings treated separately with HS and Fe; their significantly lower Fe accumulation and the slight increase of root and leaf area confirm the biostimulating effect of HS and their role in blocking Fe excess outside the roots. The use of HS can be useful for the recovery of areas contaminated by heavy metals.

Effects of humic acids from landfill leachate on plants: An integrated approach using chemical, biochemical and cytogenetic analysis.

Biological process treatment of landfill leachate produces a significant amount of sludge, characterized by high levels of organic matter from which humic acids are known to activate several enzymes of energy metabolism, stimulating plant growth. This study aimed to characterize humic acids extracted from landfill sludge and assess the effects on plants exposed to different concentrations (0.5, 1, 2 and 4 mM C L-1) by chemical and biological analysis, to elucidate the influence of such organic material and minimize potential risks of using sludge in natura. Landfill humic acids showed high carbon and nitrogen levels, which may represent an important source of nutrients for plants. Biochemical analysis demonstrated an increase of enzyme activity, especially H+-ATPase in 2 mM C L-1 landfill humic acid. Additionally, cytogenetic alterations were observed in meristematic and F1 cells, through nuclear abnormalities and micronuclei. Multivariate statistical analysis provided integration of physical, chemical and biological data. Despite all the nutritional benefits of humic acids and their activation of plant antioxidant systems, the observed biological effects showed concerning levels of mutagenicity.

Possible developments for ex situ phytoremediation of contaminated sediments, in tropical and subtropical regions - Review.

The growing problem of remediation of contaminated sediments dredged from harbor channels needs to be resolved by a cost effective and sustainable technology. Phytoremediation, by ex situ remediation plants, seems to have the potential to replace traditional methods in case of moderately contaminated sediments. On the other side, the need to mix sediments with soil and/or sand to allow an easier establishment of most employed species causes an increase of the volume of the processed substrate up to 30%. Moreover the majority of phytoremediating species are natives of temperate climate belt. Mangroves, with a special focus on the genus Avicennia - a salt secreting species - should represent an effective alternative in terms of adaptation to salty, anoxic sediments and an opportunity to develop ex situ phytoremediation plants in tropical and subtropical regions. The use of humic acid to increase root development, cell antioxidant activity and the potential attenuation of the "heavy metals exclusion strategy" to increase phytoextraction potentials of mangroves will be reviewed.

Microbiological monitoring in two areas with different levels of conservation in the mangroves of an Ecological Station, Vitoria, ES / Monitoramento microbiológico em duas áreas com diferentes níveis de conservação nos manguezais de uma Estação Ecológica, Vitória, ES

Mangroves are classified as permanent preservation areas and regarded as natural nurseries. However, they have suffered several anthropogenic stresses, resulting in their decline. In the light of that, comes the importance of researching their environmental characteristics and revealing possible factors that have led to the degradation of this important ecosystem. The aim of this study was to evaluate the environmental quality of different areas in the mangroves of Ilha do Lameirão Ecological Station through microbiological analyzes of sediment and interstitial water along ten (10) sites, distributed in two areas with different conservation levels (Canal dos Escravos (CE) and Maria Ortiz (MO)) between 2010 and 2012. The microbiological analyzes revealed that MO region, in all seasons of the year, achieved total coliform and thermo -tolerant coliform values above those permitted by the CONAMA Resolution 357/05, fitting the Class 2 conservation standard. The presence of high levels of total and thermo-tolerant coliforms in MO is a strong indicator of impacts originated from the human population and, consequently, the decline of the mangrove itself and the health of human communities surrounding that area.

Os manguezais são classificados como áreas de preservação permanente e considerados como berçários naturais, no entanto sofrem várias tensões antropogênicas, resultando em seu declínio. À luz disso, evidencia-se a importância de pesquisar suas características ambientais e reveladoras de possíveis fatores que levaram à degradação desse importante ecossistema. O objetivo deste estudo é avaliar a qualidade ambiental das diferentes áreas em manguezais da Estação Ecológica Ilha do Lameirão por meio de análises microbiológicas de sedimentos e da água intersticial ao longo de dez locais, distribuídos em duas áreas com diferentes níveis de conservação (Canal dos Escravos (CE) e Maria Ortiz (MO)) entre 2010 e 2012. As análises microbiológicas revelaram que a região MO, em todas as estações do ano, obteve valores de coliformes termo-tolerantes e totais acima do permitido pela resolução CONAMA 357/05, Classe 2. A presença de níveis elevados de coliformes totais e termo-tolerantes em MO é um forte indicador dos impactos originados da população humana e, consequentemente, do declínio do próprio mangue e da saúde das comunidades humanas que cercam a área.

Adição de ácido cítrico potencializa a ação de ácidos húmicos e altera o perfil protéico da membrana plasmática em raízes de milho / Citric acid addition improve humic acids action and change proteins profile from plasma membrane of maize roots

A promoção do crescimento vegetal pelos ácidos húmicos tem sido atribuída a ações similares a hormônios, devido à promoção do desenvolvimento e proliferação das raízes, resultando numa absorção mais eficiente de água e nutrientes. O objetivo deste trabalho foi analisar as mudanças na arquitetura radicular em plântulas de milho e no perfil de proteínas da membrana plasmática (MP) promovidas pelo tratamento com ácidos húmicos (AH) isolados de vermicomposto (20mg C L-1). O efeito da adição de ácido cítrico (AC), importante ácido orgânico presente nos exudados radiculares, sobre a bioatividade destes AH também foi investigada. Foram analisados o comprimento da raiz principal, o número de sítios de mitose, o número e comprimento de raízes laterais e a área radicular total. Para a análise do perfil protéico, vesículas da MP de células de raízes foram obtidas por fracionamento celular e as proteínas analisadas por eletroforese uni (1D) e bidimensional (2D). Observou-se que a adição de AC (0,005mM) aos AH estimularam a promoção do crescimento das raízes laterais (126 por cento), da área radicular (58 por cento) e do número de raízes laterais (55 por cento) em relação às plantas controle. A atividade da bomba de H+ da membrana plasmática, analisada como marcador bioquímico de indução do mecanismo do crescimento ácido, também foi significativamente estimulada (374 por cento) pela solução húmica suplementada com AC. O perfil protéico da MP revelou uma supressão da expressão das proteínas nesta membrana, induzida pelo tratamento com AH e, na presença de AC, esse efeito foi ainda mais evidente. Os resultados obtidos corroboram o mecanismo proposto para a bioatividade de AH no qual a ação de ácidos orgânicos exudados pelas plantas, tais como o AC, promove o rompimento da associação supramolecular dessas substâncias, tornando as moléculas bioativas presentes nos agregados húmicos mais acessíveis aos receptores celulares das raízes.

The plant growth stimulation by humic acids (HA) has been attributed to a hormone-like effect as promoting the root development and proliferation, resulting in a more efficient water and nutrient absorption. This research aims to investigate how the humic acids isolated from vermicompost (20mg L-1) can modify the root architecture and the plasma membrane (PM) protein patterns in maize roots. It was also analyzed the effect of the citric acid (CA), an organic acid present in root exudates. The changes induced in the corn root system were estimated by measuring the taproot length, the amount of root mitotic sites and lateral roots, and the total root area. Plasma membrane vesicles were purified by cell fractionation and the protein patterns were analyzed by uni (1D) and bidimensional (2D) electrophoresis. The results show that the HA in solution with CA (0.005mM) increases the lateral root growth promotion (126 percent), the root area (58 percent), and the number of lateral roots (55 percent). The activity of the plasma membrane H+ pump, analyzed as a marker of the induction of the acid growth mechanism, was also enhanced (374 percent) by the humic solution supplemented with CA. Expression of several plasma membrane proteins was inhibited when plants were treated with HA and this effect was more pronounced upon CA supplementation. The obtained results corroborate the proposed mechanism for the HA bioactivity, by which under the action of root-exuded organic acids, such as CA, a disruption of the HA macrostructure is promoted releasing bioactive molecules presented in the humic aggregates, which becomes more accessible to the root cell receptors.

Bioactivity of chemically transformed humic matter from vermicompost on plant root growth.

Chemical reactions (hydrolysis, oxidation, reduction, methylation, alkyl compounds detachment) were applied to modify the structure of humic substances (HS) isolated from vermicompost. Structural and conformational changes of these humic derivatives were assessed by elemental analyses, size exclusion chromatography (HPSEC), solid-state nuclear magnetic resonance ((13)C CPMAS-NMR), and diffusion ordered spectroscopy (DOSY-NMR), whereas their bioactivity was evaluated by changes in root architecture and proton pump activation of tomato and maize. All humic derivatives exhibited a large bioactivity compared to original HS, both KMnO(4)-oxidized and methylated materials being the most effective. Whereas no general relationship was found between bioactivity and humic molecular sizes, the hydrophobicity index was significantly related with proton pump stimulation. It is suggested that the hydrophobic domain can preserve bioactive molecules such as auxins in the humic matter. In contact with root-exuded organic acids the hydrophobic weak forces could be disrupted, releasing bioactive compounds from humic aggregates. These findings were further supported by the fact that HS and all derivatives used in this study activated the auxin synthetic reporter DR5::GUS.